Black spot corresponds to a physiological disorder of the type of oxidative stress that occurs after the prolonged postharvest storage of Persea americana Mill. cv. Hass fruit. Industry tends to confuse this disorder with pathogen attack (Colletotrichum gloeosporioides), chilling injury, mechanical damage during harvest and transport or lenticel damage. The main objectives of this research were: (i) to develop a method to assess and differentiate lenticel damage and black spot and (ii) to study the correlation between mechanical damage and lenticel damage on the development of black spot. Avocado fruits from different orchards were evaluated at two sampling times using different harvesting systems (conventional and appropriate) and at two times of the day (a.m. or p.m.). Here, we report a method based on image analysis to differentiate and quantify lenticel damage and black spot disorder. In addition, the results show that conventional harvest increased lenticel damage and lenticel damage did not correlate with black spot development but correlated with increased weight loss during prolonged postharvest storage. These results have important commercial implications since the appropriate harvesting of avocado cv. Hass would not only control the incidence of lenticel damage, which would be an advantage in terms of external quality, but also reduce weight loss during transport to distant markets.